Brand from Japan: Laguna Beaute. High quality and freshness of human adipose-derived stem cell acclimatization culture fluid.No oil, silicone, paraben (preservative), fragrance, synthetic colorant, surfactant, or prostaglandin-like substance.Triple approach with capsicum, lidensyl, and wide lash ingredients.How to use:After opening the package, put the brush down and rotate it clockwise 20-30 times (stop when liquid comes out).After the second time, keep the brush down and rotate it clockwise until liquid comes out, and apply morning and evening from the base of the lashes before makeup.